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array tm ifc  (fluidigm)


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    Structured Review

    fluidigm array tm ifc
    Array Tm Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/Assay+Loading+Reagent/pm39855563-97-8-14
    Average 93 stars, based on 148 article reviews
    array tm ifc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Circulating microRNAs Showed Specific Responses according to Metabolic Syndrome Components and Sex of Adults from a Population-Based Study
    Article Snippet: The product of Reverse Transcription was then diluted 1/10, and 1.25 μL of diluted cDNA was preamplified using Fluidigm PreAmp Master mix (Paris, France, Cat. 100-5580) and primer mix in 5 μL reaction volume according to the supplier’s specifications. .. For each assay, qPCR was performed in triplicate from 1.5 μL of diluted preamplification product (1/10) using Biotium Fast Probe Master Mix (Fremont, CA, Cat. 310005) in dynamic arrays 96 × 96 IFC (Cat. BMK-M-96.96) on a Biomark TM instrument (Fluidigm, Paris, France) according to the supplier’s protocol. ..

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly
    Article Snippet: .. In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles. ..

    Article Title: Evaluation of Human Bone Marrow Mesenchymal Stromal Cell (MSC) Functions on a Biomorphic Rattan-Wood-Derived Scaffold: A Comparison between Cultured and Uncultured MSCs
    Article Snippet: The cDNA was pre-amplified using 14 cycles and PA master mix using Fluidigm (Fluidigm, San Fransisco, CA, USA) reagents as per the manufacturer’s protocols for scaffolds established from cultured MSCs or BMAs, respectively. .. Quantitative polymerise chain reaction (qPCR) was then performed on a Fluidigm 48.48 Dynamic Array TM integrated fluid circuit (IFC, Fluidigm) using HPRT1 and as the housekeeping gene. ..

    other:

    Article Title: Association between Germline Single-Nucleotide Variants in ADME Genes and Major Molecular Response to Imatinib in Chronic Myeloid Leukemia Patients
    Article Snippet: Selected gSNVs significantly associated with response to imatinib ( p < 0.05) in the discovery set were then tested in an independent cohort of 137 CP-CML patients using the 96.96 Dynamic Array TM integrated fluidic circuit (IFC) with SNP Type Assay chemistry on the Biomark HD system (Fluidigm, South San Francisco, CA, USA), with previous pre-amplification of all the DNA samples and following the manufacturer’s instructions.

    High Throughput Screening Assay:

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly
    Article Snippet: .. In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles. ..

    Nucleic Acid Electrophoresis:

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly
    Article Snippet: .. In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles. ..

    Polymerase Chain Reaction:

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly
    Article Snippet: .. In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles. ..

    Article Title: Novel translational mouse models of Metabolic Dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity.
    Article Snippet: For amplification, we used the PreAmp 13 master Mix (Fluidigm, PN 100-5580) according to the manufacture guidelines (Real-Time PCR user 14 guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used 15 the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading 16 Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master 17 Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..

    Article Title: Novel translational mouse models of metabolic dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity
    Article Snippet: For amplification, we used the PreAmp master Mix (Fluidigm, PN 100–5580) according to the manufacture guidelines (Real-Time PCR user guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..

    Amplification:

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly
    Article Snippet: .. In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles. ..

    Gene Expression:

    Article Title: Novel translational mouse models of Metabolic Dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity.
    Article Snippet: For amplification, we used the PreAmp 13 master Mix (Fluidigm, PN 100-5580) according to the manufacture guidelines (Real-Time PCR user 14 guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used 15 the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading 16 Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master 17 Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..

    Article Title: Novel translational mouse models of metabolic dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity
    Article Snippet: For amplification, we used the PreAmp master Mix (Fluidigm, PN 100–5580) according to the manufacture guidelines (Real-Time PCR user guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..



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    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
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    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
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    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
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    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
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    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose genotyping. The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose genotyping. The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Clone Assay, Whole Genome Amplification, Derivative Assay, Software, Amplification, Expressing, Real-time Polymerase Chain Reaction

    Genotyping by Fluidigm BioMarkTM IFC Dynamic ArrayTM quantitative PCR. (A) WGA-FLDMqPCR: WGA-amplified DNA and qPCR. Left: double-strand DNA (dsDNA) accumulation curve as a function of the number of cycles. Right: melting curve of the final product. Green: positive control (duck DNA). Red: a hybrid which was positive (containing duck DNA corresponding to the marker tested). Blue: a negative hybrid. Yellow: negative control (hamster DNA). (B) Pre-ampFLDMqPCR: non-amplified DNA, a pre-amplification step with a mix of the 96 primer pairs for the 96 markers tested in the Fluidigm BioMarkTM assay and qPCR. The same markers and controls are used as in (A) . The sensitivity is higher in (B) , with a lower number of cycles necessary for detection of duck DNA. The negative control and the hybrid not containing duck DNA amplify at a much higher number of cycles and the non-specific products amplified can easily be distinguished by their different melting temperature values (right). In both experiments, no amplification was obtained from water (data not shown).

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Genotyping by Fluidigm BioMarkTM IFC Dynamic ArrayTM quantitative PCR. (A) WGA-FLDMqPCR: WGA-amplified DNA and qPCR. Left: double-strand DNA (dsDNA) accumulation curve as a function of the number of cycles. Right: melting curve of the final product. Green: positive control (duck DNA). Red: a hybrid which was positive (containing duck DNA corresponding to the marker tested). Blue: a negative hybrid. Yellow: negative control (hamster DNA). (B) Pre-ampFLDMqPCR: non-amplified DNA, a pre-amplification step with a mix of the 96 primer pairs for the 96 markers tested in the Fluidigm BioMarkTM assay and qPCR. The same markers and controls are used as in (A) . The sensitivity is higher in (B) , with a lower number of cycles necessary for detection of duck DNA. The negative control and the hybrid not containing duck DNA amplify at a much higher number of cycles and the non-specific products amplified can easily be distinguished by their different melting temperature values (right). In both experiments, no amplification was obtained from water (data not shown).

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Positive Control, Marker, Negative Control

     Genotyping  8 no hit markers using three different genotyping strategies

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Genotyping 8 no hit markers using three different genotyping strategies

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques:

    Comparison of marker retention with the three  genotyping  techniques

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Comparison of marker retention with the three genotyping techniques

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Marker